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Swine Flu's Back and Badder than Ever: Recombination and G158E Duplication in H1N1 Iowa Swine
07-09-2010, 04:13 AM,
#1
Bug  Swine Flu's Back and Badder than Ever: Recombination and G158E Duplication in H1N1 Iowa Swine
Quote:Recombination and G158E Duplication In H1N1 Iowa Swine
Recombinomics Commentary 16:50
July 6, 2010

The recently released HA sequence from A/swine/Iowa/03032/2010 has a six BP (Base Pairs) duplication which includes G158E. Thus instead of the wild type sequence for positions 156-159 changing from KKGN to KKEN, as found in many low reactors, the Iowa swine sequence has KKEKEN, which almost certainly leads to significant immunological escape.

The isolate was collected a month ago and the genetic background is similar to humans infected by swine H1N1 in 2007 in Ohio. However, the sequence has also acquired pH1N1 sequences that are found in almost all pH1N1 isolates. Moreover, most of these polymorphisms are non-synonymous and at least one polymorphism traces back to 1918.

These acquisitions raise concerns that this virus could quickly gain efficient and sustained transmission in humans, leading to a co-circulating H1N1 pandemic.

More intense screening of swine in the United States would be useful.
http://www.recombinomics.com/News/07061001/G158E_Dup.html

Influenza A virus (A/swine/Iowa/03032/2010(H1N1)) hemagglutinin (HA) gene, complete cds
GenBank: HM585495.1
http://www.ncbi.nlm.nih.gov/nuccore/299766147?report=GenBank

In short a mutated version of H1N1 has appeared in the US and was submitted to a Minnesota veterinary lab on 24-JUN-2010 where it was confirmed on 03-JUL-2010. With the introduction of the pH1N1 sequences it can transmit much more quickly to and within the human populace. It is not compatible for 'defence' with the old vaccine and a new batch of chemical cocktail will be brewed for the herd.
There are no others, there is only us.
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07-09-2010, 02:32 PM,
#2
RE: Swine Flu's Back and Badder than Ever: Recombination and G158E Duplication in H1N1 Iowa Swine
I'll pass on the chemical cocktail, I've already had my quota of who knows what injected into me.
An error does not become truth by reason of multiplied propagation, nor does truth become error because nobody sees it.
Mohandas Gandhi


Each of us is put here in this time and this place to personally decide the future of humankind.
Did you think you were put here for something less?
Chief Arvol Looking Horse
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07-09-2010, 09:05 PM,
#3
RE: Swine Flu's Back and Badder than Ever: Recombination and G158E Duplication in H1N1 Iowa Swine
"It is not compatible for 'defence' with the old vaccine and a new batch of chemical cocktail will be brewed for the herd. "
The fact that all the old vaccines have expired by now means that PhARMA needs to say the new flu can't be counteracted by old immunizations so they can create demand for a new one.
Just end factory farming and this won't be as much of an issue.
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07-10-2010, 01:47 AM,
#4
RE: Swine Flu's Back and Badder than Ever: Recombination and G158E Duplication in H1N1 Iowa Swine
Different, more transmissible and fatal flavours have been showing up in (are being released to) the wild since April.

Severe pH1N1 Cases In West Georgia Raise Pandemic Concerns
http://www.issapharma.org/blog,severe-ph1n1-cases-in-west-georgia-raise-pandemic-concerns,227219.html

Widespread pH1N1 Tamiflu Resistance In South Korea
http://www.issapharma.org/blog,widespread-ph1n1-tamiflu-resistance-in-south-korea,233491.html

pH1N1 D225G In 2010 North Carolina and Wyoming Sequences
http://www.issapharma.org/blog,ph1n1-d225g-in-2010-north-carolina-and-wyoming-sequences,228594.html

Widespread pH1N1 Low Reactors In Europe
http://www.issapharma.org/blog,widespread-ph1n1-low-reactors-in-europe,244676.html

Hong Kong D225G Limited To Severe and Fatal pH1N1
http://www.issapharma.org/blog,hong-kong-d225g-limited-to-severe-and-fatal-ph1n1,228943.html

D225G and Swine Acquisitions in India pH1N1 Case
http://www.issapharma.org/blog,d225g-and-swine-acquisitions-in-india-ph1n1-case,250513.html

But getting back to the initial Iowa Swine seems the source were pigs from the county fair and it was spread to humans..

Quote:Parallel H1N1 Pandemics in 2010?
Recombinomics Commentary 03:50
July 7, 2010

An H1N1 influenza A virus, A/swine/Ohio/24366/07, was isolated from pigs in an Ohio county fair. Twenty-six people who came in contact with the infected pigs developed respiratory disease and two of these people were laboratory confirmed as H1N1 by the Centers for Disease Control and Prevention (CDC). The A/swine/Ohio/24366/07 virus we isolated from swine was shown at the CDC to have 100% identical genome sequence to the human virus associated with the county fair.

The above comments describe a swine H1N1 isolate from pigs at a 2007 Huron county fair in Ohio. In 2009 the sequences from the two confirmed cases, an exhibitor (10F) and her father (36M) were released. The two human HA sequences, A/Ohio/01/2007 and A/Ohio/02/2007, were identical to each other and from a triple reassortant with a human PB1, avian PB2 and PA and 5 North American swine sequences for the other five gene segments.

Recently a 2010 swine sequence from Iowa was released, A/swine/Iowa/03032/2010. It was most closely related to the two 2007 human sequences, but had acquired a number of non-synonymous polymorphisms found in 2009/2010 pH1N1 isolates including a duplication of G158E, a change associated with low reactors.

These developments have raised concerns of a second H1N1 pandemic.

The 2007 isolates almost caused a pandemic in 2007, based on the large number of fair attendees who developed respiratory symptoms in August, and the confirmation of swine H1N1 in the two cases described above. However, the lack of efficient transmission prevented the identification of additional cases.

The 2007 sequences had wild type sequences at positions 156-159 which represented an antigenic site that has led to immunological escape in 2009 pH1N1 isolates, while the 2010 isolate from Iowa has G158E, as well as a duplication of this polymorphism to produce a sequences of KKEKEN at the antigenic site, which will likely significantly blunt the effect of pre-existing antibodies directed against this site.

Prior 2010 swine sequences demonstrated pH1N1 transmission from swine to swine in the United States and G158E frequencies are increasing. This transmission in swine has led to the acquisition of a number of polymorphisms by the Iowa sequence, raising concerns that the new acquisitions, coupled with changes and duplication at position 158, will create a new H1N1 that is efficiently transmitted in humans, which would create distinct, but co-circulating pH1N1 species in humans.

The acquisition of the pH1N1 polymorphisms by swine H1N1 has remarkable parallels with the 1918 pandemic. The 1918 sequence have alternating regions of Iowa swine and human H1N1 polymorphisms generated by recombination. The similar evolution in 2010 Iowa swine sequences is cause for concern. Moreover, since pH1N1 and Iowa swine H1N1 share polymorphisms, distinctions may require sequencing, which is currently at a low level. An increase in swine and human surveillance, including sequencing, are dictated by the striking acquisitions by the swine sequence from Iowa.
http://www.issapharma.org/blog,parallel-h1n1-pandemics-in-2010,299356.html
There are no others, there is only us.
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07-10-2010, 04:12 AM, (This post was last modified: 07-10-2010, 04:12 AM by h3rm35.)
#5
RE: Swine Flu's Back and Badder than Ever: Recombination and G158E Duplication in H1N1 Iowa Swine
Yawn wake me when it's over...
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